Actin Dynamics During Phagocytosis
(Quicktime video, coming soon)

Macrophage (J774.1) is taking up two pH-sensitive-fluorescein-labeled zymosans (green).
(Video Clip, avi, 5.27MB)

GFP-expressing salmonella (green) moving inside a macrophage with the nucleus stained with HE (hydroethidine)(red).
(Video Clip, avi, 14.2MB)

Fusion data from J774 macrophage cells (A) and from alveolar macrophages (B). Intracellular zymosan (green) fused with lysosomes (red) appear yellow.

Dynamin (green) does not colocalize with most peripheral polymerized actin ruffles (red) in J774 macrophages

Dynamin (green) does not colocalize with giantin in the cis-Golgi(red) in J774 cells.

Quantal release of superoxide (red) coupled with membrane capacitance increase (blue).

Fluorescein-labeled zymosan (green) is squenched in Cftr^+/+ , but in Cftr^-/- the ability to acidify is defective resulting in the internalized fluorescent-green zymosan.

Ultrastructrural analysis of hydrophobic polystyrene bead uptake in J774 macrophages. 

Cells were exposed to 0.8 :m IgG-coated polystyrene beads 20 min prior to fixation.  A capacitance trace following the uptake of beads was shown in D.

Lysotracker positive vesicles (red) in alveolar macrophages.

Free radical production (green) in the phagosome in bone marrow macrophage fed with zymosan.  

Comparison of lysotracker positive vesicles (red) per cell in the.antisense transfected cells (A) to sense transfected cells (B). Green: transfection marker.

Schematic representation of the coupled experimental measurement of changes in membrane capacitance accompanying the secretory response and amperometric measurement of free radical (superoxide release) and changes in internal calcium following release of caged calcium (NP-EGTA) by a UV based flash lamp .